Scientific Program

Day 1 :

Keynote Forum

Juergen Lademann

Director Center of Experimental and Applied Cutaneous Physiology, Germany

Keynote: Drug Delivery by Nanoparticles

Time : 10:00-10:45

Biography:

Jurgen Lademann graduated from the Moscow Lomonosov State University in 1980. 1991 he received the Assistant Professorship from the University of Jena, Germany. From 1993 to 1995 he headed the Medical Center of Sensor Technology at the University of the Armed Forces Germany. In 1996 Jurgen Lademann joined the Charite Universitatsmedizin Berlin and was appointed Director of the Center of Experimental and Applied Cutaneous Physiology. In 2001 the Charite appointed him full professor of Dermatology. He is the Vice President of the IFSCC and the editor of the Journal Skin Pharmacology and Physiology. He authored more than 600 peer-reviewed articles.

Abstract:

The demands on nanoparticles for use in dermatology and cosmetics are very different. While nanoparticles widely applied in sunscreens, like TiO2 and ZnO, shall remain on the skin surface or in the upper cell layers of the stratum corneum, nanoparticles intended for drug delivery shall penetrate through the skin barrier to the target structures in the living cells.

At the Charité - Universitätsmedizin Berlin various laser scanning microscopy methods are used to investigate the penetration and storage of nanoparticles in the skin, hair follicles being in the focus of attention. Human hair follicles are ideal target structures for drug delivery. Hosting both the stem and dendritic cells, they are surrounded by a dense network of blood vessels. Investigating nanoparticles of different size and materials, it was found that particles of approximately 600nm diameter penetrate most efficiently into the hair follicles and can be stored there for ca. 10 days. Their retention time in the hair follicles exceeds that in the stratum corneum by almost one order of magnitude.

Keynote Forum

Michael J O Rourke

President Scotia Vision Consultants, USA

Keynote: The Development and Commercialisation of Sustained Release Ocular Drug Delivery Systems

Time : 10:45-11:25

Biography:

Michael O'Rourke has over 30 years drug delivery experience across ophthalmology, periodontal and pulmonary markets in sales, marketing, product launch, strategy development and global commercialization. In 2009 he founded Scotia Vision, a specialized ophthalmic consulting company with extensive expertise in global ocular drug delivery commercial and product development strategies. Career experience includes senior positions with several global leading organizations and start-ups, including 3M, Alza, Chiron Vision, Bausch + Lomb, GrayBug and Re-Vana Therapeutics. His unique global ocular drug delivery experience includes launching the world’s first intra ocular drug delivery technology in Europe, Vitrasert, and the world’s second, Retisert, in the USA. He’s managed 28 brands, led 13 product launches, structured/negotiated 12 strategic business deals and has been a team member in 18 device/drug approvals. Michael is from Glasgow Scotland but is now based in Tampa Florida. He was recently elected into the Global Scot "Hall of Fame”, for supporting and advising Scottish start ups and existing organisations in the Life Sciences and other sectors including music with the Royal Scottish National Orchestra.

Abstract:

The global ophthalmic pharmaceutical industry is estimated to reach $29 billion by 2022 with a growing elderly population and the increasing incidence of diabetic eye disease, due in part to increasing obesity rates in both adults and children. The number of people visually impaired in the world is approx. 295 million, with at least 39 million blind and 256 million having low vision. 65% of people visually impaired and 82% of all blind are 50 years and older. There is urgent need to develop new therapeutics and ocular drug delivery systems, providing controlled release for 4-6 months or longer for the treatment of chronic and blinding eye diseases. These will increase patient' s and doctor' s convenience by reducing the dosing frequency including the ability to minimize frequent intra vitreal injections for chronic conditions of the retina. Currently there are only 4 sustained release ocular drug delivery systems ever approved worldwide, treating blinding eye diseases. There is a significant need for both new delivery systems and therapeutics for both small and large molecules. This presentation will cover the development of systems to date, discuss new technologies under current development, the challenges to overcome and how they can ultimately successfully commercialized.

  • Green Bio-analysis
Speaker
Biography:

Ahmed Mamun has worked in the career of pharmaceutical industry for 11 years, in the field of analytical chemistry, methodology and validation. Since 5 years he is a Quality control supervisor in orchidia pharmaceuticals for ophthalmic.He has been a researcher in analytical chemistry of faculty of pharmacy cairo university since 2012. He is the Editorial board member in Asian journal of Lifesciences.

 

Abstract:

In recent years, the whole field of ion-selective electrodes(ISEs) in pharmaceutical sciences has expanded far beyond its original roots. The diverse range of opportunities offered by ISEs was broadly used in a number of pharmaceutical applications, with topics presented ranging from bioanalysis of drugs and metabolites, to protein binding studies, green analytical chemistry, impurity profiling, and drug dissolution in biorelevant media. Inspired from these advances and with the aim of extending the functional capabilities of ISEs, the primary focus of the present paper is the utilization of ISE as a tool in personalized medicine. Given the opportunity to explore biological events in real-time (such as drug metabolism) could be central to personalized medicine. (ATR) is a chemo-degradable and bio-degradable pharmaceutically active drug. Laudanosine (LDS) is the major degradation product and metabolite of ATR and is potentially toxic and reported to possess epileptogenic activity which increases the risk of convulsive effects. In this work, ATR have been subjected to both chemical and biological hydrolysis, and the course ofthe reactions is monitored by means of a ISE. In this study, we have designed an efficient real-time tracking strategy which substantially resolve the challenges of the ATR chemical and biological degradation kinetics. By utilizing a potentiometric sensor, tracking of ATR chemical and biological degradation kinetics can be performed in a very short time with excellent accuracy. The LOD was calculated to be 0.23 mol L−1, the potential drift was investigated over a period of 60 min and the value was 0.25 mVh−1. Real serum samples for measurement the rate of in vitro metabolism of ATR was performed. Furthermore, a full description of the fabricated screen-printed sensor was presented. 

  • Targeted Drug delivery

Session Introduction

Surangi Jayawardena

Assistant professor The University of Alabama in Huntsville USA

Title: Inflamed site-specific drug delivery system using human serum albumin nanoparticles
Speaker
Biography:

Abstract:

The work demonstrates the use of carbohydrate-conjugated nanomaterial (glyconanomaterial) to target bacterial pathogens. A collection of systematic studies done by several groups has demonstrated that bacteria can be specifically targeted using various oligosaccharides. Through our preliminary investigations we have discovered that oligosaccharide conjugated nanoparticles (glyconanoparticles) could be used to target strain specific bacteria. For example D-maltoheptaose (G7) and trehalose conjugated nanoparticle used to effectively target Escherichia coli and Mycobacterium spp. Antibiotic resistance in pathogenic bacterial strains is a growing global concern. We have demonstrated that these glyconanoparticles have been used as a carrier for antibiotics that would help target bacteria and reduce minimum inhibitory concentration of a conventional antibiotic. Antibiotic streptomycin (Str) is a broad range aminoglycoside typically used in the treatment of tuberculosis. We have bi-functionalized a nanoparticle using carbohydrate-G7 antibiotic-Str to produce glyconanoantibiotics (GNAs). As proof of concept for active targeting GNAs are used against a highly Str resistant E. coli strain. The GNAs demonstrated size dependent increased antibacterial efficacy few log folds improvement over the free the free antibiotic (Str).

Yasunori Iwao

Associate Professor School of Pharmaceutical Sciences,University of Shizuoka, Japan

Title: Inflamed site-specific drug delivery system using human serum albumin nanoparticles
Speaker
Biography:

Abstract:

To develop a new strategy for inflamed site-specific drug delivery in the colon for the treatment of ulcerative colitis (UC), we focused on the interaction between myeloperoxidase (MPO) and human serum albumin (HSA) and prepared nanoparticles (HSA NPs) conjugated with 5-aminosalicylic acid (5-ASA), an anti-inflammatory drug. The 5-ASA-HSA NPs (nine molecules of 5-ASA per HSA molecule) were uniform particles with an average particle size of 190 nm, a zeta potential of -11.8 mV, and a polydispersity index of 0.35. This was considered a suitable particle characteristic to pass through the mucus layer and accumulate into the mucosa. The specific interaction between the 5-ASA-HSA NPs and MPO was observed using quartz crystal microbalance analysis in vitro. In addition, the 5-ASA-HSA NPs group containing one thousandth of the dose of the 5-ASA (75 μg/kg) showed significantly lower disease activity index values and colon weight/length ratios in UC model mice as similar to large amount of neat 5-ASA group (75 mg/kg), indicating that the therapeutic effect of the 5-ASA-HSA NP formulation was confirmed in vivo. Microscopic images of tissue sections of colon extracted from UC model mice demonstrated that HSA NPs and MPO were both localized in the colon, and this specific interaction between HSA NPs and MPO would be involved the in the therapeutic effect in vivo. Furthermore, in the 5-ASA and 5-ASA-HSA NPs groups, some inflammatory damage was observed in the colon, but the degree of damage was mild compared with the control and HSA NPs groups, suggesting mucosal repair and replacement with fibrous granulation tissue had occurred. Therefore, these data demonstrated for the first time, that an HSA NP formulation has the potential to specifically deliver 5-ASA to an inflamed site where MPO is highly expressed.

  • Chemical Analysis

Session Introduction

Jayalakshmi Sridhar

Assistant Professor Xavier University of Louisiana, LA, USA

Title: Discovery of a synthetic method to form 2,2 - bis(naphthoquinones) compounds
Speaker
Biography:

Jayalakshmi Sridhar has her expertise in organic synthesis of complex molecules and computational molecular modeling. Her research work group focuses on the development of modulators for three classes of proteins - (1) protein kinases that regulate many of the key signaling pathways in the cell, (2) the cytochrome P450 enzymes that metabolize exogenous and endogenous substances in the body and (3) Liver X Receptor b that is a key mediator in many of the cellular processes. The development of new synthetic methods for the target molecule is part of the research focus. The discovery of one such reaction trais a practical, efficient, and transition metal free approach to the synthesis of binaphthyltetraones using Diels-Alder reaction of substituted trimethylsilane dienes with benzoquinone.

Abstract:

Quinones are key structural components in many natural products, and therapeutic drugs impacting many disease conditions, viz antimicrobial, antiparasitic, anti-tumor, inhibition of PGE2 biosynthesis and anti-cardiovascular disease. As part of our efforts in the design and development of kinase inhibitors as potential therapeutics for cancer and Alzheimer’s disease, the synthesis of monohydroxynaphthoquinones using Diels-Alder reaction was pursued. This lead to the discovery of a novel method for the synthesis of 2,2’-bis(naphthoquinones)  using conjugated ketene silyl acetals with benzoquinone. The analysis of the reaction conditions and the product structures using NMR and X-ray lead to the elucidation of a credible mechanism of dimer formation. The control of reactant stoichiometry to yield either the monomer of the dimer product is explored. The results of the study of this new synthetic method are presented.

 

  • Application of polymers for drug delivery

Session Introduction

Mariusz Skwarczynski

Associate Professor The University of Queensland, St. Lucia, Queensland, Australia

Title: Polyacrylate-based Vaccine Delivery System
Speaker
Biography:

Abstract:

Classical vaccines incorporating live or attenuated microorganisms possess several disadvantages and cannot be applied against cancer and some pathogens. Modern vaccines utilizing immunogenic subunits derived from a particular pathogen are able to overcome these obstacles but need a specific delivery system for their efficacy. Nanotechnology has opened a new window into these delivery methodologies. Particles-based subunit vaccine formulations have been proven to be very effective in inducing cellular and humoral immune responses.

We synthesized polymeric constructs consisting of polyacrylate cores and peptide epitopes derived from bacterial or cancer antigens. The peptide epitopes were synthesized using solid phase peptide synthesis, whereas polymeric cores were synthesized by successive atom transfer radical polymerization. Unprotected peptide epitopes containing an N-terminus azide moiety were conjugated to core structures via copper-catalyzed alkyne-azide 1,3-dipolar cycloaddition “click” reaction. The self-adjuvanting vaccine particles were formed under aqueous conditions and purified by dialysis. The particles were characterized by dynamic light scattering, transmission electron microscopy and elemental analysis to determine size and conjugation efficacy between polymer and peptides. Ability of nanoparticles to induce humoral immune responses against Group A Streptoccocus was examined in mice. To test the efficacy of polymer-peptide conjugates as a therapeutic vaccine against established tumors, in vivo tumor treatment experiments were performed based on well-established procedures with C57BL/6 mice using TC-1 tumor model.

The designed new delivery system based on the polyacrylate polymer with self-assembled properties induced humoral immune responses which were dependent on the particle size. The strong immune responses were observed even after single subcutaneous immunization. The produced antibodies were able to recognize and kill clinical isolated bacterial strains. The polymer-peptide conjugates were also used for the design of therapeutic vaccine against cervical cancer. Treatment with these conjugates led to significantly better survival compared to treatment with any other immunogens including IFA-adjuvanted positive control. Our findings suggested that this delivery system is a promising strategy for the design of prophylactic vaccines to induce humoral immunity as well as therapeutic peptide-based vaccines that induce adequate cellular immunity against a target disease. This delivery system also removes the use of incompletely defined and ordinarily toxic immune adjuvants, producing a safe and effective for potential vaccines for human use.

  • Herbal drug formulations and evaluations

Session Introduction

Rohit P. Dugar

Formulation Scientist Nutrilite Health Institute, Amway Greater Los Angeles Area

Title: Effect of granulation and tableting process parameters on microbial bio-burden in tablets containing plant based ingredients: A case study
Speaker
Biography:

Abstract:

Strict guidelines are enforced by governing bodies of different countries for the APC of dietary and natural products. In the current study, effect of processing parameters on microbial bio-burden is explored. Effect of process parameters of high shear (HS) and fluidized top spray (TS) granulation on microbial bio-burden of tablets containing natural products was studied. Components of formulation were accurately weighed and blended using a V-blender to prepare 6 kg batches each for HS and TS processes. 24full factorial design was created using Minitab® as shown in Table 1. High shear wet granulation was carried out in a GEA PMA™ using starch slurry in de-ionized water. Wet mass was passed through a co-mill and then dried in a fluidized bed drier until constant LOD values were obtained. Fluidized top spray granulation was carried out in a GEA MP1™. Inlet air temperature and velocity were modified as per Table 1. Post drying, granules were milled at two different speeds and then compressed on a rotary tablet press at two different main compression forces. Aerobic plate count (APC) swabs, loss on drying and water activity were performed before each processing stage through final tableting. Initial dry blend APC was an average 50000 CFU/g. Figure 1 shows a 1.3-fold decrease in APC after the milling step post high shear granulation while a 3.5-fold kill after the drying step in fluidized bed drier. Post tableting, a 47-fold APC decrease was observed for low compression forces while a 50-fold for high forces. High rate of microbial kill during tableting could be attributed to the high shear forces and localized heating while the press is running. This work takes a deep dive into identification and optimization of each processing step involved in tableting in regard to the microbial load of the final formulations.

  • Use of Nanoparticles in DDS and newer methodologies
Speaker
Biography:

Abstract:

It is of utmost importance to deliver protein in safe and efficacious form from the nano particulate delivery system knowing that adsorption of proteins on to nanoparticle surfaces involves complex interactions including hydrophobic interaction, electrostatic interaction and/or interaction between adsorbed protein molecules which may lead to loss of protein stability. However, adsorption based drug delivery systems for protein drugs would be one of the simplest and most effective forms of drug if the mechanistic understanding of adsorption of proteins at solid/liquid interfaces is obtained. Evaluation of the changes in structure of recombinant human growth hormone (r-hGH) upon adsorption at biodegradable Poly (lactide-co-glycolide)PLGA nanoparticles of different hydrophobicity as a function of pH revealed the polymer grades suitable for delivery system.  The comprehension of structural stability and polymer grade is extremely useful in developing sustained delivery of protein like r-hGH. This kind of dosage form is currently lacking in the market despite the facts that r-hGH was first approved for use by FDA in 1995, the conventional dosage form in the market has a limitation of daily subcutaneous injections, a long-acting dosage form of r-hGH; Nutropin depot was discontinued in 2004, about 5 years after its approval from FDA and since then continued research has been focused in this area. Thus, direct evaluation of secondary and tertiary structural conformations of the adsorbed proteins on the polymer surface with the analytical techniques such as dynamic light scattering Spectroscopy, fluorescence spectroscopy and circular dichroism spectroscopy will help understand the nature of the interactions that govern the adsorption of the protein on the polymer, leading to successful design of nano particulate delivery systems.

  • Developments in Drug Delivery
Speaker
Biography:

Abstract:

Purpose: To prepare a new EGCG delivery system by utilizing nano-EGCG loaded thermoreversible in situ hydrogel to enhance EGCG stability and resident time in adipose tissue ablation.  The present study may poetically provide a better way to achieve a maximum therapeutically benefit of nano-EGCG on abdominal obesity treatment.

Methods: Nano-EGCG was prepared using a phase inversion-based process by encapsulating EGCG into chitosan coated nanostructured lipid carriers. Nano-EGCG was then dispersed into thermoreversible Pluronic® F127-based hydrogel by cold method. The sol-gel transition temperatures were evaluated using inverted tube method. The stability of native EGCG, nano-EGCG solution, nan-EGCG loaded gel were investigated in 1×PBS (pH=7.4) at 37°C for 15 days.  In vitro EGCG release from nano-EGCG loaded in situ gel were detected by a membrane-less diffusion method. In vitro cytotoxicity and cellular triglyceride accumulation in 3T3-L1 adipocytes cells were determined by MTT and an enzymatic method, respectively.

  • Vaccine Design and Drug Delivery Technology

Session Introduction

Istvan Toth

Professor The University of Queensland, St. Lucia, Queensland, Australia

Title: Lipophilic vaccine delivery systems
Speaker
Biography:

Abstract:

Infection with group A streptococci (Streptococcus pyogenes, GAS), one of the common and widespread human pathogens, can result in a broad range of diseases, with the potential of acute and post-infectious rheumatic fever and rheumatic heart disease. Immunity to GAS relies on the production of opsonic antibodies specific to the hypervariable N-terminal and conserved C-terminal regions of the coiled-coil α-helical M protein, the major virulent factor in GAS. The development of an effective vaccine for GAS has been challenged by the induced autoimmunity of epitopes derived from the C-terminal regions, unsuitable B-cell epitopes that have been shown to react with human heart tissue, and the minimal B-cell epitopes, which believed to be safe, shows little or no immunogenicity unless bound to a delivery platform. For vaccine delivery, self-adjuvanting lipid core peptide (LCP) and polymer coated liposome systems including antigen, carrier and adjuvant within the same molecular entity has been developed. The systems allow the attachment of multiple copies of antigens.
 
Methods
We synthesized a dendritic structure (LCP) consisting of a lipoamino acids, polylysine carrier and a peripheral generation of the minimal B-cell epitope (J14) and CD4+ T helper cell epitope (P25). The peptide dendritic core and the epitopes were synthesized using solid phase peptide synthesis. Blank liposomes which contained the LCP were formulated and optimized for charge and lipid content using a thin film formation method. Optimized liposomes were coated with positively charged trimethyl chitosan (TMC) then negatively charged sodium alginate in a layer-by-layer approach. These formulations were subsequently characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Optimized formulations were further investigated for their efficiency of uptake by intestinal immune cells and ability to induce mucosal IgA and systemic IgG responses after oral administration.

  • Novel Drug Delivery System

Session Introduction

Gozde Kabay

TOBB University of Economics and Technology, Turkey

Title: Melanin Functionalized Protein Nanofibers: A Bio-inspired Platform for Drug Release
Speaker
Biography:

Abstract:

A recent approach for controlled release of drugs comprise natural-derived biopolymers since they show superior biodegradable/biocompatible features. In this study, nanofibers containing natural melanin and amyloid-like bovine serum albumin (mel/BSA) were produced by single-needle electrospinning to investigate drug release behavior of water soluble model drug, namely ampicillin. Prior to electrospinning, natural melanin was extracted from cuttle fish ink to prepare electrospinning solution. 20%, 10% and %5 (w:w) ampicilling:BSA including nanofibers were produced under 21 kV, 0.3 ml/h for 4 hours. Nanofiber formation and morphology for different drug formulations was observed by scanning electron microscope. Fourier transform infrared spectroscopy analysis showed that some compatibility exists between ampicillin and mel/BSA nanofibers. Moreover, drug release tests were conducted to explain the relationship between the drug release behavior and carrier morphology. For single electrospun nanofibers, 87% of the loaded drug was released within the 96 hours. Together, these imply that drug release vehicles could be extended to melanin functionalized biopolymer carriers.

Speaker
Biography:

Abstract:

The eye is an intricate organ, it consists of many barriers that help to protect and maintain its normal function. It is made up of two segments, the anterior segment – which includes the cornea, ciliary body, conjunctiva, sclera, and anterior uvea – and the posterior segment – which includes the retina and vitreous body. The protective design of the eye makes delivery of drugs to the back of the eye (posterior segment) difficult. However, with an ageing population, there is an increased number of blinding conditions that occur in the posterior segment that requires more effective and long-acting drug delivery systems (Mashaghi et al., 2016; Marshal, 1987).

Day 2 :

Keynote Forum

Robert Lee

Executive Vice President Particle Sciences USA

Keynote: Development and Characterization of Sterile Nanoparticulate Suspensions

Time : 10:00-10:45

Biography:

Dr. Lee is Executive Vice President of Pharmaceutical Development Services at Particle Sciences, a Lubrizol Advanced Materials, Inc. company, a pharmaceutical contract development and manufacturing organization (www.particlesciences.com). He provides direction and is involved with product and business development. Before joining Particle Sciences, Rob held senior management positions at Novavax, Inc., Lyotropic Therapeutics, Inc., and Imcor Pharmaceutical Co. He holds BSs in Biology and Chemistry from the University of Washington and a PhD in Physical Bioorganic Chemistry from the University of California, Santa Barbara. Rob has published articles in numerous peer-reviewed journals and five book chapters plus holds over two dozen issued patents or provisional patent applications.

Abstract:

TBA

Biography:

Behzad Nili has completed his PharmD/PhD at the age of 27 years from Turin University, School of Pharmcey, Italy and thought at SUMS Shiraz University School of Medical Sciences, in both Departments of Pharmaceutics and Medicinal Chemistry, for several years. He has been working for Nano Drug Delivery, since 2004

Abstract:

TBA

Keynote Forum

Vladimir Matveevich Gruznov

Deputy Director of Trophimuk Institute of petroleum geology and geophysics Russia

Keynote: State of the art in chemical-analytical methods for the detection of explosives

Time :

Biography:

Vladimir Matveevich Gruznov, Doctor of Sciences (Chromatography, Geophysics), Science Deputy Director of Trophimuk Institute of petroleum geology and geophysics Siberian Branch of  Russian Academy of Sciences (Novosibirsk). Field of work:  substance and elemental analysis for explosives detection, geophysics and ecological monitoring. Publications: over 190 research papers including 3 monographs and 16 patents.

 

Abstract:

TBA

  • Electrophoresis

Session Introduction

Elif Tugce AKSUN TUMERKAN

Researcher, University of Exeter, College of Life and Environmental Sciences,United Kingdom

Title: The Comparison of Different Gel Composition and Staining Agents for gel electrophoresis of Fluorescent Protein
Speaker
Biography:

Elif  Tugce AKSUN TUMERKAN  has her expertise in  proteomic and biotechnology. Her studies generally based on gel electrophoresis, fluorescent protein and their potential uses in cell biology.She is doing research related to fluorescent protein from marine organisms. And potential approach in different cell tissue.

 

Abstract:

Fluorescent proteins are unique in that they are self-sufficient in forming chromophores with a visible wavelength from 3 amino acids sequence within their own polypeptide structure. The development of fluorescent proteins (FPs) and their applications is an outstanding example of basic science leading to practical biotechnological and medical applications. Fluorescent proteins have several applications in science and are used as important indicators in molecular biology and cell-based research, and as effective biosensors. While these proteins occur in numerous marine species, FPs from anthozoans are the best-understood models for experimental cell biology. For clarifying functional properties of fluorescent protein, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis is used essentially. In protein- based research, many technique are used for determining functionality of protein, however, SDS-PAGE analysis can only provide a molecular level assessment of the proteolytic fragments. In this study, variations of fluorescent protein band mobility compared based on resolving gel composition and for determination of visualizing of protein band, different staining agents evaluated. For this aim, fluorescent proteins were obtained from the symbiotic sea anemone (Anemonia viridis) tentacles and   protein bands determined which depend on molecular weight by SDS-PAGE analysis. For SDS-PAGE analysis, protein bands run 3 different composition of resolving gels (7.5, 10 and 12%). After gel electrophoresis process the protein bands are visualized with Coomassie brilliant blue staining and silver nitrate. Then the gels are then destained in KCl. The results showed that variations in protein migration with slightly different depend on resolving gel structure. The best mobility property was found in 12% resolving gel. For visualising, the coomassie brilliant blue was found the best staining agent. Depend on obtained result, it can be pointed out that gel structure and staining procedure have influence on fluorescent protein gel band quality and visibility