Molecular Biology and Microbiology Techniques

Molecular techniques can form the basis of remote instrumentation sensing technologies for marine microbial diversity and ecological function. Here we review some of the most commonly used molecular biological techniques. These techniques include the polymerase chain reaction (PCR) and reverse transcriptase PCR, quantitative PCR, whole assemblage “fingerprinting” approaches (based on nucleic acid sequence or length heterogeneity), oligonucleotide microarrays, and high-throughput shotgun sequencing of whole genomes and gene transcripts, which can be used to answer biological, ecological, evolutionary and biogeochemical questions in the ocean sciences

Microbiology techniques are methods used for the study of microbes, including bacteria and microscopic fungi and protists. They include methods to survey, culture, stain, identify, engineer and manipulate microbes. Microbiology is the study of microorganisms including viruses, bacteriafungi, and protozoa. Microbiological aspects are very important to humans because many diseases are caused by microorganisms. Microbiology techniques include agar diffusion test, ATP test, bacterial inhibition assay, CAMP test, endospore staining, indole test, microbiological culture, etc. Moreover, microbiology also helps to develop many industrial applications with the help of microorganisms, for example; bakery industry, the pharmaceutical industry, beer industry, etc.

  • Track 1-1 Isolation of Nucleic Acids
  • Track 2-2 Nucleic Acid Hybridization
  • Track 3-3 DNA Analysis
  • Track 4-4 Staining Techniques

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