Basic Principles of Chromatography

Chromatography is the most powerful separation technique available. For reasons that it is easy to implement chromatographic techniques with small samples, and to carry out separations on an analytical scale. Separated components are usually detected online, and sometimes they are also characterized, after which they are generally discarded. In preparative applications, the purified components are collected for further investigation, and operate at high concentrations. Chromatography is a separation method based on differences in equilibrium constants for the components of a mixture placed in a diphasic system.


  • Track 1-1 Gas Chromatography
  • Track 2-2 Thin Layer Chromatography
  • Track 3-3 Liquid Chromatography

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