Electrophoresis

As an analytical tool electrophoresis is unsophisticated, swift and highly fragile. It is used analytically to study the properties of a single charged species and as a separation technique. It provides the basis for a number of analytical techniques used for separating molecules by size, charge, or binding affinity, example- for the separation of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or protein molecules using an electric field applied to a gel matrix. Gel matrix used mainly is polyacrylamide and agarose. DNA Gel electrophoresis is usually carried out for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or southern blotting for further characterization.

  • Track 1-1 Affinity Electrophoresis
  • Track 2-2 Capillary Electrophoresis
  • Track 3-3 Immunoelectrophoresis
  • Track 4-4 Pulsed field Gel Electrophoresis
  • Track 5-5 Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
  • Track 6-6 Native Gels
  • Track 7-7 Electrofocusing Gels
  • Track 8-8 DNA Agarose Gels

Related Conference of Chemistry